Adjonu, R., Doran, G., Torley, P., & Agboola, S. (2013). Screening of whey protein isolate hydrolysates for their dual functionality: Influence of heat pre-treatment and enzyme specificity. Food Chemistry, 136(3–4), 1435–1443. https://doi.org/10.1016/j.foodchem.2012.09.053
Amigo-Benavent, M., & FitzGerald, R. J. (2022). Impact of thermal inactivation conditions on the residual proteolytic activity and the viscosity properties of whey protein concentrate enzymatic hydrolysates. Food Hydrocolloids, 124. https://doi.org/10.1016/j.foodhyd.2021.107333
Antošová, A., Gancar, M., Bednarikova, Z., Marek, J., Bystrenová, E., & Gažová, Z. (2022). The influence of cations on Α-lactalbumin amyloid aggregation. Jbic Journal of Biological Inorganic Chemistry, 27(7), 679–689. https://doi.org/10.1007/s00775-022-01962-3
Besharati, M., & Lackner, M. (2023). Bioactive peptides: a review. In Eurobiotech Journal (Vol. 7, Issue 4, pp. 176–188). Sciendo. https://doi.org/10.2478/ebtj-2023-0013
Bhat, Z. F., Morton, J. D., El-Din A. Bekhit, A., Kumar, S., & Bhat, H. F. (2021). Processing technologies for improved digestibility of milk proteins. In Trends in Food Science and Technology (Vol. 118, pp. 1–16). Elsevier Ltd. https://doi.org/10.1016/j.tifs.2021.09.017
Boyle, R. J., Ierodiakonou, D., Khan, T., Chivinge, J., Robinson, Z., Geoghegan, N., Jarrold, K., Afxentiou, T., Reeves, T., Cunha, S., Trivella, M., Garcia-Larsen, V., & Leonardi-Bee, J. (2016). Hydrolysed formula and risk of allergic or autoimmune disease: systematic review and meta-analysis. BMJ (Clinical Research Ed.), 352, i974. https://doi.org/10.1136/bmj.i974
Butré, C. I., Sforza, S., Gruppen, H., & Wierenga, P. A. (2014). Determination of the influence of substrate concentration on enzyme selectivity using whey protein isolate and bacillus licheniformis protease. Journal of Agricultural and Food Chemistry, 62(42), 10230–10239. https://doi.org/10.1021/jf503151f
Cheison, S. C., & Kulozik, U. (2017). Impact of the environmental conditions and substrate pre-treatment on whey protein hydrolysis: A review. Critical Reviews in Food Science and Nutrition, 57(2), 418–453. https://doi.org/10.1080/10408398.2014.959115
Choudhary, U., Poonia, A., & Iñiguez-Moreno, M. (2023). Whey: Chemistry and Its Biotechnological Potential. In A. Poonia & A. Trajkovska Petkoska (Eds.), Whey Valorization (pp. 31–48). Springer Nature Singapore. https://doi.org/10.1007/978-981-99-5459-9
Chourasia, R., Phukon, L. C., Abedin, M. M., Padhi, S., Singh, S. P., & Rai, A. K. (2022). Whey valorization by microbial and enzymatic bioprocesses for the production of nutraceuticals and value-added products. In Bioresource Technology Reports (Vol. 19). Elsevier Ltd. https://doi.org/10.1016/j.biteb.2022.101144
Clouzot, H., Thibodeau, J., Bazinet, L., Froidevaux, R., & Mikhaylin, S. (2025). Pulsed electric field pretreatment of bovine α-lactalbumin: Effect of processing parameters on structure, hydrolysis and production of potential antihypertensive and antidiabetic peptides. Food and Bioproducts Processing, 153, 43–57. https://doi.org/10.1016/j.fbp.2025.04.004
Corrochano, A. R., Ferraretto, A., Arranz, E., Stuknytė, M., Bottani, M., O’Connor, P. M., Kelly, P. M., De Noni, I., Buckin, V., & Giblin, L. (2019). Bovine whey peptides transit the intestinal barrier to reduce oxidative stress in muscle cells. Food Chemistry, 288, 306–314. https://doi.org/10.1016/j.foodchem.2019.03.009
Cruz-Casas, D. E., Aguilar, C. N., Ascacio-Valdés, J. A., Rodríguez-Herrera, R., Chávez-González, M. L., & Flores-Gallegos, A. C. (2021). Enzymatic hydrolysis and microbial fermentation: The most favorable biotechnological methods for the release of bioactive peptides. Food Chemistry: Molecular Sciences, 3. https://doi.org/10.1016/j.fochms.2021.100047
Dalkiran, A., Rifaioglu, A. S., Martin, M. J., Cetin-Atalay, R., Atalay, V., & Doğan, T. (2018). ECPred: a tool for the prediction of the enzymatic functions of protein sequences based on the EC nomenclature. BMC Bioinformatics, 19(334), 1–13. https://doi.org/10.1186/s12859-018-2368-y
Daroit, D. J., & Brandelli, A. (2021). In vivo bioactivities of food protein-derived peptides – a current review. Current Opinion in Food Science, 39, 120–129. https://doi.org/10.1016/j.cofs.2021.01.002
Duvaud, S., Gabella, C., Lisacek, F., Stockinger, H., Ioannidis, V., & Durinx, C. (2021). Expasy, the Swiss Bioinformatics Resource Portal, as designed by its users. Nucleic Acids Research, 49, 216–227. https://doi.org/10.1093/nar/gkab225
Eberhardt, A., López, E. C., Marino, F., Mammarella, E., Manzo, R. M., & Sihufe, G. A. (2021). Whey protein hydrolysis with microbial proteases: determination of kinetic parameters and bioactive properties for different reaction conditions. International Journal of Dairy Technology, 74(3), 489–504. https://doi.org/10.1111/1471-0307.12795
Fadimu, G. J., Le, T. T., Gill, H., Farahnaky, A., Olatunde, O. O., & Truong, T. (2022). Enhancing the Biological Activities of Food Protein-Derived Peptides Using Non-Thermal Technologies: A Review. In Foods (Vol. 11, Issue 13). MDPI. https://doi.org/10.3390/foods11131823
Figueroa, O. A., Alean, J. D., Marcet, I., Rendueles, M., & Zapata, J. E. (2023). Kinetic-conceptual model of the hydrolysis of bovine plasma proteins - ALCALASE® 2.9L: The role of inhibition by product. Heliyon, 9(10), e21002. https://doi.org/10.1016/j.heliyon.2023.e21002
FitzGerald, R. J., Cermeño, M., Khalesi, M., Kleekayai, T., & Amigo-Benavent, M. (2020). Application of in silico approaches for the generation of milk protein-derived bioactive peptides. Journal of Functional Foods, 64(October 2019), 103636. https://doi.org/10.1016/j.jff.2019.103636
Gammoh, S., Alu’datt, M. H., Alhamad, M. N., Alrosan, M., Al-Husein, B., Al-u’datt, D., Alkandari, S., Rababah, T., Ammari, Z., Albiss, B., Alzoubi, H., & Kubow, S. (2022). Enzymatic bioactive peptides from sonicated whey proteins of camel milk: impacts of nanopeptides on structural properties, antioxidant activity and inhibitory activity of Alpha-amylase and ACE. International Journal of Dairy Technology, 75(4), 791–802. https://doi.org/10.1111/1471-0307.12890
Gao, R., Kong, F., Mu, G., Zhao, X., Cheng, J., & Qian, F. (2025). Screening of enzymes for bi-functional whey protein hydrolysates: virtual enzymolysis, fragmentomics, and molecular docking in silico. Food Research International, 214, 116629. https://doi.org/10.1016/j.foodres.2025.116629
García, J. M., Herrera-Rocha, F., Cavajalino, A. S., Barón, R. D., Barrios, A. F. G., & Udenigwe, C. C. (2021). Effects of Processing Conditions on Hydrolysates of Proteins From Whole Whey and Formation of Maillard Reaction Products. Journal of Food Processing and Preservation, 45(9), 15469. https://doi.org/10.1111/jfpp.15469
Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M. R., Appel, R. D., & Bairoch, A. (2005). Protein Identification and Analysis Tools on the ExPASy Server. In J. M. Walker (Ed.), The Proteomics Protocols Handbook (pp. 571–607). Humana Press. https://doi.org/10.1385/1-59259-890-0:571
Gaviria, Y. S., Gómez-Sampedro, L., Zapata, J. E., Miedes, D., Cilla, A., & Alegría, A. (2024). Iron chelation capacity and prediction of peptide binding modes identified in California red worm hydrolysates and effects on iron bioavailability in differentiated Caco-2 cells. Food Bioscience, 62, 105023. https://doi.org/10.1016/j.fbio.2024.105023
Gómez, L. J., & Zapata, J. E. (2022). Caracterización fisicoquímica, tecnofuncional y calidad biológica de hidrolizados de vísceras de Tilapia Roja (Oreochromis spp.). Información Tecnológica, 33(3), 3–14. https://doi.org/10.4067/s0718-07642022000300003
Habinshuti, I., Nsengumuremyi, D., Muhoza, B., Ebenezer, F., Yinka Aregbe, A., & Antoine Ndisanze, M. (2023). Recent and novel processing technologies coupled with enzymatic hydrolysis to enhance the production of antioxidant peptides from food proteins: A review. In Food Chemistry (Vol. 423). Elsevier Ltd. https://doi.org/10.1016/j.foodchem.2023.136313
Hayes, M., Aluko, R. E., Aurino, E., & Mora, L. (2023). Generation of bioactive peptides from porphyridium sp. and assessment of their potential for use in the prevention of hypertension, inflammation and pain. Marine Drugs, 21(8), 422. https://doi.org/10.3390/md21080422
Hayes, M., Mora, L., & Lucakova, S. (2022). Identification of Bioactive Peptides from Nannochloropsis oculata Using a Combination of Enzymatic Treatment, in Silico Analysis and Chemical Synthesis. Biomolecules, 12(12). https://doi.org/10.3390/biom12121806
Iskandar, M. M., Lands, L. C., Sabally, K., Azadi, B., Meehan, B., Mawji, N., Skinner, C. D., & Kubow, S. (2015). High hydrostatic pressure pretreatment of whey protein isolates improves their digestibility and antioxidant capacity. Foods, 4(2), 184–207. https://doi.org/10.3390/foods4020184
Kleekayai, T., O’neill, A., Clarke, S., Holmes, N., O’sullivan, B., & Fitzgerald, R. J. (2022). Contribution of Hydrolysis and Drying Conditions to Whey Protein Hydrolysate Characteristics and In Vitro Antioxidative Properties. Antioxidants, 11(2). https://doi.org/10.3390/antiox11020399
Koirala, P., Dahal, M., Rai, S., Dhakal, M., Nirmal, N. P., Maqsood, S., Al-Asmari, F., & Buranasompob, A. (2023). Dairy Milk Protein-Derived Bioactive Peptides: Avengers Against Metabolic Syndrome. Current Nutrition Reports, 12(2), 308–326. https://doi.org/10.1007/s13668-023-00472-1
Landim, A. P. M., Chávez, D. W. H., Rosa, J. S. da, Mellinger-Silva, C., & Rosenthal, A. (2021). Effect of high hydrostatic pressure on the antioxidant capacity and peptic hydrolysis of whey proteins. Ciência Rural, 51(4). https://doi.org/10.1590/0103-8478cr20200560
Landim, A. P. M., Matsubara, N. K., da Silva-Santos, J. E., Mellinger-Silva, C., & Rosenthal, A. (2022). Application of preliminary high-pressure processing for improving bioactive characteristics and reducing antigenicity of whey protein hydrolysates. Food Science and Technology International, 28(6), 489–501. https://doi.org/10.1177/10820132211022106
Le Maux, S., Nongonierma, A. B., Barre, C., & Fitzgerald, R. J. (2016). Enzymatic generation of whey protein hydrolysates under pH-controlled and non pH-controlled conditions: Impact on physicochemical and bioactive properties. Food Chemistry, 199, 246–251. https://doi.org/10.1016/j.foodchem.2015.12.021
Le Maux, S., Nongonierma, A. B., & FitzGerald, R. J. (2017). Peptide composition and dipeptidyl peptidase IV inhibitory properties of β-lactoglobulin hydrolysates having similar extents of hydrolysis while generated using different enzyme-to-substrate ratios. Food Research International, 99, 84–90. https://doi.org/10.1016/j.foodres.2017.05.012
Le Maux, S., Nongonierma, A. B., Lardeux, C., & FitzGerald, R. J. (2018). Impact of enzyme inactivation conditions during the generation of whey protein hydrolysates on their physicochemical and bioactive properties. International Journal of Food Science and Technology, 53(1), 219–227. https://doi.org/10.1111/ijfs.13576
Liao, W., Sun, G., Xu, D., Wang, Y., Lu, Y., Sun, J., Xia, H., & Wang, S. (2021). The Blood-Pressure-Lowering Effect of Food-Protein-Derived Peptides: A Meta-Analysis of Recent Clinical Trials. In Foods (Vol. 10, Issue 10, p. 2316). https://doi.org/10.3390/foods10102316
López, E. C., Eberhardt, A., Marino, F., Mammarella, E., Sihufe, G. A., & Manzo, R. M. (2022). Physicochemical Characterisation of ACE-inhibitory and Antioxidant Peptides From Alcalase® Whey Protein Hydrolysates Using Fractionation Strategies. International Journal of Dairy Technology, 75(3), 538–552. https://doi.org/10.1111/1471-0307.12872
Mann, B., Athira, S., Sharma, R., Kumar, R., & Sarkar, P. (2019). Bioactive peptides from whey proteins. In Whey Proteins: From Milk to Medicine (pp. 519–547). Elsevier. https://doi.org/10.1016/B978-0-12-812124-5.00015-1
Mazorra-Manzano, M. A., Robles-Porchas, G. R., González-Velázquez, D. A., Torres-Llanez, M. J., Martínez-Porchas, M., García-Sifuentes, C. O., González-Córdova, A. F., & Vallejo-Córdoba, B. (2020). Cheese whey fermentation by its native microbiota: Proteolysis and bioactive peptides release with ACE-inhibitory activity. Fermentation, 6(1), 1–12. https://doi.org/10.3390/fermentation6010019
Minkiewicz, P., Iwaniak, A., & Darewicz, M. (2019). BIOPEP-UWM database of bioactive peptides: Current opportunities. International Journal of Molecular Sciences, 20(23). https://doi.org/10.3390/ijms20235978
Minkiewicz, P., Iwaniak, A., & Darewicz, M. (2022). BIOPEP-UWM Virtual—A novel database of food-derived peptides with in silico-predicted biological activity. In Applied Sciences (Vol. 12, Issue 14, p. 7204). https://doi.org/10.3390/app12147204
Moriyama, Y., Kondo, N., & Takeda, K. (2012). Secondary structural changes of homologous proteins, lysozyme and Α-lactalbumin, in thermal denaturation up to 130 °C and sodium dodecyl sulfate (SDS) effects on these changes: Comparison of thermal stabilities of SDS-induced helical structures in these p. Langmuir, 28(47), 16268–16273. https://doi.org/10.1021/la3035598
Murtaza, M. A., Irfan, S., Hafiz, I., Ranjha, M. M. A. N., Rahaman, A., Murtaza, M. S., Ibrahim, S. A., & Siddiqui, S. A. (2022). Conventional and Novel Technologies in the Production of Dairy Bioactive Peptides. In Frontiers in Nutrition (Vol. 9). Frontiers Media S.A. https://doi.org/10.3389/fnut.2022.780151
Nielsen, S. D. H., Liang, N., Rathish, H., Kim, B. J., Lueangsakulthai, J., Koh, J., Qu, Y., Schulz, H. J., & Dallas, D. C. (2023). Bioactive milk peptides: an updated comprehensive overview and database. Critical Reviews in Food Science and Nutrition, 64(31), 11510–11529. https://doi.org/10.1080/10408398.2023.2240396
Nong, N. T. P., & Hsu, J. L. (2022). Bioactive Peptides: An Understanding from Current Screening Methodology. In Processes (Vol. 10, Issue 6). MDPI. https://doi.org/10.3390/pr10061114
Nongonierma, A. B., Paolella, S., Mudgil, P., Maqsood, S., & FitzGerald, R. J. (2018). Identification of novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in camel milk protein hydrolysates. Food Chemistry, 244, 340–348. https://doi.org/10.1016/j.foodchem.2017.10.033
Olsen, T. H., Yesiltas, B., Marin, F. I., Pertseva, M., García-Moreno, P. J., Gregersen, S., Overgaard, M. T., Jacobsen, C., Lund, O., Hansen, E. B., & Marcatili, P. (2020). AnOxPePred: using deep learning for the prediction of antioxidative properties of peptides. Scientific Reports, 10(1), 1–10. https://doi.org/10.1038/s41598-020-78319-w
Olvera-Rosales, L. B., Cruz-Guerrero, A. E., García-Garibay, J. M., Gómez-Ruíz, L. C., Contreras-López, E., Guzmán-Rodríguez, F., & González-Olivares, L. G. (2023). Bioactive peptides of whey: obtaining, activity, mechanism of action, and further applications. In Critical Reviews in Food Science and Nutrition (Vol. 63, Issue 30, pp. 10351–10381). Taylor and Francis Ltd. https://doi.org/10.1080/10408398.2022.2079113
Osman, A., El-Hadary, A., Korish, A. A., AlNafea, H. M., Alhakbany, M. A., Awad, A. A., & Abdel-Hamid, M. (2021). Angiotensin-I converting enzyme inhibition and antioxidant activity of papain-hydrolyzed camel whey protein and its hepato-renal protective effects in thioacetamide-induced toxicity. Foods, 10(2), 1–11. https://doi.org/10.3390/foods10020468
Ostertag, F., & Hinrichs, J. (2023). Enrichment of lactoferrin and immunoglobulin G from acid whey by cross-flow filtration. Foods, 12(11), 2163. https://doi.org/10.3390/foods12112163
Ozorio, L., Pereira, N. R., da Silva-Santos, J. E., Brígida, A. I. S., Mellinger-Silva, C., & Cabral, L. M. C. (2019). Enzyme inactivation and drying technologies influencing the vasorelaxant activity of a whey protein hydrolysate in semi-pilot scale. International Dairy Journal, 93, 11–14. https://doi.org/10.1016/j.idairyj.2019.01.016
Öztürk, H. İ., & Oraç, A. (2024). Harvesting bioactive peptides from sustainable protein sources: Unveiling technological and functional properties through in silico analyses. Food and Humanity, 2(April), 100294. https://doi.org/10.1016/j.foohum.2024.100294
Pillai, A. T., Morya, S., & Kasankala, L. M. (2024). Emerging trends in bioavailability and pharma-nutraceutical potential of whey bioactives. Journal of Nutrition and Metabolism, 2024. https://doi.org/10.1155/2024/8455666
Rajput, Y. S., & Sharnna, R. (2023). Enzynnes Beyond Traditional Applications in Dairy Science and Technology. Academic Press Inc.
Rama, G. R., Kaufmann, E. S., Ducati, R. G., Camargo de Lima, J., Staats, C., Timmers, L. F. S. M., & Volken de Souza, C. F. (2024). From in silico to in vitro: Hydrolysis of cheese whey to obtain peptides with anti-aging and anti-hypertensive activities. Biocatalysis and Agricultural Biotechnology, 57, 103093. https://doi.org/10.1016/j.bcab.2024.103093
Rani, S., Pooja, K., & Pal, G. K. (2018). Exploration of rice protein hydrolysates and peptides with special reference to antioxidant potential: Computational derived approaches for bio-activity determination. Trends in Food Science and Technology, 80(November 2017), 61–70. https://doi.org/10.1016/j.tifs.2018.07.013
Rivero-Pino, F., Millan-Linares, M. C., & Montserrat-de-la-Paz, S. (2023). Strengths and limitations of in silico tools to assess physicochemical properties, bioactivity, and bioavailability of food-derived peptides. Trends in Food Science and Technology, 138(June), 433–440. https://doi.org/10.1016/j.tifs.2023.06.023
Sedaghati, M., Ezzatpanah, H., Boojar, M. M.-A., Ebrahimi, M., & Aminafshar, M. (2014). Plasmin-digest of Β-lactoglobulin with antibacterial properties. Food and Agricultural Immunology, 26(2), 218–230. https://doi.org/10.1080/09540105.2014.893998
Selvaggi, M. (2015). Β-lactoglobulin gene polymorphisms in sheep and effects on milk production traits: a review. Advances in Animal and Veterinary Sciences, 3(9), 478–484. https://doi.org/10.14737/journal.aavs/2015/3.9.478.484
Sereda, A. S., Kostyleva, E. V., Velikoretskaya, I. A., & Tsurikova, N. V. (2022). Whey proteins hydrolysis using Alcalase and Flavourzyme. IOP Conference Series: Earth and Environmental Science, 1052(1). https://doi.org/10.1088/1755-1315/1052/1/012045
Sharma, R. K., Furusawa, K., Fukui, A., Sasaki, N., & Masalova, I. (2018). FTIR spectroscopic study of heat-induced Β-lactoglobulin solution under flow field. Asian Journal of Chemistry, 30(6), 1321–1324. https://doi.org/10.14233/ajchem.2018.21232
Tu, M., Cheng, S., Lu, W., & Du, M. (2018). Advancement and prospects of bioinformatics analysis for studying bioactive peptides from food-derived protein: Sequence, structure, and functions. TrAC Trends in Analytical Chemistry, 105, 7–17. https://doi.org/10.1016/j.trac.2018.04.005
Ulug, S. K., Jahandideh, F., & Wu, J. (2021). Novel technologies for the production of bioactive peptides. In Trends in Food Science and Technology (Vol. 108, pp. 27–39). Elsevier Ltd. https://doi.org/10.1016/j.tifs.2020.12.002
Vidal-Limon, A., Aguilar-Toalá, J. E., & Liceaga, A. M. (2022). Integration of molecular docking analysis and molecular dynamics simulations for studying food proteins and bioactive peptides. Journal of Agricultural and Food Chemistry, 70(4), 934–943. https://doi.org/10.1021/acs.jafc.1c06110
Wang, B., Meng, T., Ma, H., Zhang, Y., Li, Y., Jin, J., & Ye, X. (2016). Mechanism study of dual-frequency ultrasound assisted enzymolysis on rapeseed protein by immobilized Alcalase. Ultrasonics Sonochemistry, 32, 307–313. https://doi.org/10.1016/j.ultsonch.2016.03.023
Wei, G., Wang, T., Li, Y., He, R., Huang, A., & Wang, X. (2024). Identification, structural characterization, and molecular dynamic simulation of ACE inhibitory peptides in whey hydrolysates from Chinese Rushan cheese by-product. Food Chemistry: X, 21(February), 101211. https://doi.org/10.1016/j.fochx.2024.101211
Yang, X., Ren, X., & Ma, H. (2022). Effect of Microwave Pretreatment on the Antioxidant Activity and Stability of Enzymatic Products from Milk Protein. Foods, 11(12). https://doi.org/10.3390/foods11121759
Yiğit, A., Bielska, P., Cais-Sokolińska, D., & Samur, G. (2023). Whey proteins as a functional food: Health effects, functional properties, and applications in food. Journal of the American Nutrition Association, 42(8), 758–768. https://doi.org/10.1080/27697061.2023.2169208
Zhang, Y., Aryee, A. N., & Simpson, B. K. (2020). Current role of in silico approaches for food enzymes. Current Opinion in Food Science, 31, 63–70. https://doi.org/10.1016/j.cofs.2019.11.003
Zhang, Y., Liu, Y., Li, W., & Han, W. (2024). Antioxidant activity of soybean peptides and Keap1 protein: A combined in vitro and in silico analysis. LWT, 212, 117019. https://doi.org/10.1016/j.lwt.2024.117019
Zhu, X., Xie, D., Zhu, Q., Li, Y., & Cui, C. (2023). Preparation of Β-lactoglobulin-derived tryptophan peptide and its effect on anxiety-like behaviors in zebrafish. Frontiers in Nutrition, 9, 1100718. https://doi.org/10.3389/fnut.2022.1100718
